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人胎盘催乳素(HPL)

更新时间:2021-05-10

简要描述:

上海恒过生物长期代理美国进口分装、原装ELISA“人胎盘催乳素(HPL)",现货供应,价格优惠,质量保证。中英文双版说明书,公司提供免费代测服务。欢迎您来电来涵.

型号:HY21327E点击量:150

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试剂名称:

人胎盘催乳素(HPL)

英文名称:Human placenta lactogen,HPL ELISA KIT

检测范围:详见说明书

产品规格:96人份/48人份

检测目标:人、大小鼠、猪、豚鼠、鸡、羊、兔...

保存条件: 2-8℃

应用范围:科研用检测试剂

人胎盘催乳素(HPL)相关产品:
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Assay procedure(操作步骤)
1.Dilute and add sample:Dilute Original density Standard as follow table:
8 pg/ml 5 Standard 150μl Original density Standard+150μl Standard diluent
4pg/ml 4 Standard 150μl 5 Standard+150μl Standard diluent
2 pg/ml 3 Standard 150μl 4 Standard+150μl Standard diluent
1 pg/ml 2 Standard 150μl 3 Standard +150μl Standard diluent
0.5 pg/ml 1 Standard 150μl 2 Standard +150μl Standard diluent
2.add sample:Set blank wells separay (blank comparison wells don’t add sample and HRP-
Conjugate reagent, other each step operation is same). testing sample well. add Sample
dilution 40μl to testing sample well, then add testing sample 10μl (sample final
dilution is 5-fold), add sample to wells , don’t touch the well wall as far as
possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold wash solution diluted 30-fold (or 20-fold) with distilled
water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer
to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the
light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color
change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution
and within 15min. 

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