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恒远生物|鱼指标elisa文献分享

更新时间:2023-05-08   点击次数:289次

上海恒远生物科技有限公司专注生命科学和生物医学领域十一年,是集研发、生产、销售于一体的专业生物公司。公司具有完善的实验技术开发实验室,成熟的抗体研发系统,熟练掌握各种生物学技术,结合公司的研发团队,可以有效的保证公司产品具有强的稳定性和高的准确性,同时又具有竞争力的价格。公司目前可以提供多种ELISA检测试剂盒、细胞、进口常规生化试剂、抗体、化学试剂、药典级试剂、标准品、血清等产品,覆盖分子生物学、生物化学、细胞生物学、免疫学、蛋白组学、生物制药与诊断试剂研发生产等生命科学的各个领域。

 

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恒远产品文献:5HT3A,AChR,DRD5等引用文献

【文献标题】Abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudates

【作者】Wenwen Cai , Hugh J. MacIsaac , Runbing Xu , et.al

【作者单位】云南大学(Yunnan University

【文献中引用产品】

科研用鱼5-羟色胺受体3a5HT3AELISA试剂盒

科研用鱼乙酰胆碱受体(AChR)ELISA试剂盒

科研用鱼胆碱乙酰转移酶(CHATELISA试剂盒

科研用鱼多巴脱羧酶 (DDC)  ELISA试剂盒

科研用鱼多巴胺受体 DRD5ELISA试剂盒

科研用鱼β-2肾上腺素能受体(ADRβ2)ELISA试剂盒

科研用鱼钠依赖性去甲肾上腺素转运体(SLC6A2ELISA试剂盒

科研用鱼钠-葡萄糖协同转运蛋白1(SGLT1)ELISA试剂盒

科研用鱼苯乙醇胺-n-甲基转移酶(pnmt)ELISA试剂盒

科研用鱼多巴胺β-单加氧酶(dbmoELISA试剂盒

科研用鱼酪氨酸酶(tyrELISA试剂盒

科研用鱼c-fos ELISA试剂盒

【影响因子(IF)7.12

【出版期刊】《Ecotoxicology and Environmental Safety

【产品原文引用】

All target receptors/enzymes in this study were tested by Fish Enzyme Linked Immunosorbent Assay Kit made against Salmo salar (Solarbio Life Science, Beyotime Biotechnology and Hengyuan Biotechnology). We measured 5-hydroxytryptamine receptor 3A-like (5HT3A), acetylcholine receptor (AchR), acetylcholinesterase (AchE), acetylcholine transferase (ChAT), sodium- and chloride-dependent GABA transporter 1-like (GAT1), aromatic-L-amino-acid decarboxylase (DDC), D (5)-like dopamine receptor (DRD5), beta-2 adrenergic receptor-like (ADRβ2), sodium-dependent noradrenaline transporterlike (SLC6A2), sodium/glucose cotransporter 1-like(SGLT1),phenylethanolamine N-methyltransferase (PNMT), dopamine betamonooxygenase (DBMO), tyrosinase (TYR), and proto-oncogene (cfos). All tests were performed according to kit instructions with three biological replicates. Because antibodies used in the kits were not designed for our fish, the golden line barbel, it may underestimate the concentration due to low cross reactivity. Potassium and sodium concentration were measured with atomic absorption spectroscopy.

 

恒远产品文献:G6Pase,FBPase,胰岛素,GLP-1 ELISA试剂盒引用文献

【文献标题】Effects of dietary mannan oligosaccharides (MOS) supplementation on metabolism, inflammatory response and gut microbiota of juvenile Nile tilapia (Oreochromis niloticus) fed with high carbohydrate diet

【作者】Tong Wang , Hong-Xia Wu, Wei-jie Li, et.al

【作者单位】华东师范大学(East China Normal University

【文献中引用产品】

鱼葡萄糖6磷酸酶/葡萄糖6磷酸酯酶(G6Pase)ELISA试剂盒

鱼果糖-1,6-二磷酸酶(FBPase)ELISA试剂盒

鱼胰岛素(Insulin)ELISA试剂盒

鱼胰高血糖素样肽1(GLP-1)ELISA试剂盒

【关键词】Nile tilapia (Oreochromis niloticus) ,Mannan oligosaccharides ,High carbohydrate ,Metabolism disorders ,Inflammation ,Intestinal microbiota

【影响因子(IF)4.62

【出版期刊】《Fish and Shellfish Immunology

【产品原文引用】

The activities of glucose-6-phosphatase (G6pase, HB985-QT) and fructose- 1,6-diphosphate (FBPase, HB984-QT) were assayed by commercial kits (Shanghai Hengyuan Biological Technology Co.,Ltd, China). The frozen samples of liver were homogenized (dilution 1:10) in ice-cold buffer. The homogenate was centrifuged at 2500 rpm for 10 min at 4 ?C. The supernatant was separated and used for measurement of enzymes activity according to the manufacturers protocols.

 

Enzyme-linked immunosorbent assay (ELISA) kits were used to measure plasma insulin (HB407-QT) and glucagon-like peptide 1 (GLP-1, HB299-QT) levels (Shanghai Hengyuan Biological Technology Co., Ltd, China). All experiments were conducted according to the relevant kit protocols.

 

 

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