补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子及 ECM 表达的影响
郑孟君1 ,曹建民2 ,郭 娴2 ,周海涛3
(1. 江南大学 体育学院,江苏 无锡 214000;2. 北京体育大学 运动人体科学学院,北京 100084;
3. 北京联合大学 生物化学工程学院,北京 100023)
摘 要: 观察补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子及 ECM 表达的影响。方法:75 只 7 周龄雄性Wistar 大鼠随机分为 4 组:安静对照组( C 组,12 只) 、一般训练组( M 组,12 只) 、过度训练组( OM 组,24 只) 和补益中药 + 过度训练组( TM 组,24 只) ,M、OM、TM 组进行 8 周 56 天的游泳训练。采用专业灌胃器每天灌胃 1 次,TM 组灌胃采用黄芪、红景天、丹参、苦参等组成的复合中药制剂,剂量为 1 g·kg - 1 ,体积为 5 mL·kg - 1 ,其他各组灌胃等量生理盐水。末次训练后 24 h,采用 PAS 染色观察肾小球 ECM 沉积情况,免疫组织化学法检测肾组织 TNF-α、IL- 1β、IL-6、IL-18 蛋白表达,RT-PCR 法检测肾组织 TNF-α mRNA、IL-1β mRNA、IL-6 mRNA、IL-18 mRNA、TGF-β1 mRNA表达。结果:1)8 周实验后,肾小球 ECM 沉积,C、M 组间无显著差异( P > 0. 05 );OM 组较 C、M 组显著增加( P <
0. 01);TM 组显著低于 OM 组( P < 0. 05)。2) 血尿素氮和血清肌酐水平,OM 组和 TM 组显著高于 C 组( P < 0. 01), TM 组显著低于 OM 组( P < 0. 05);肾组织 TNF-α、IL-1β、IL-6 和 IL-18 蛋白表达,OM 组和 TM 组显著高于 C 组( P <
0. 01) ,TM 组显著低于 OM 组( P < 0. 05);肾组织 TNF-α mRNA、IL-1β mRNA、IL-6 mRNA 和 IL-18 mRNA 表达,OM、 TM 组显著高于 C 组( P < 0. 01) ,TM 组显著低于 OM 组( P < 0. 05);肾组织 TGF-β1mRNA 表达,TM 组( P < 0. 05) 和 OM 组( P < 0. 01) 显著高于C 组,TM 组显著低于OM 组( P < 0. 05)。结论:8 周过度训练致大鼠发生运动性肾缺血再灌注,同时 ECM 沉积加强。补充补益中药可通过抑制肾脏组织 TNF-α、IL-1β、IL-6、IL-18 的表达进而减轻了过度训练诱导肾脏缺血再灌注发生时肾脏组织 TGF-β1 的表达,抑制 ECM 的合成和( 或) 促进 ECM 的降解及组织病理学改变,延缓或避免过度训练导致的运动性缺血再灌注对肾脏的损害。
关键词: 补益中药;运动性肾缺血再灌注损伤;炎症因子;细胞外基质
中图分类号: G804. 7 文献标志码: A 文章编号: 1004 - 0560( 2015) 03 - 0068 - 06
Effect of Tonic Medicine on Kidney Inflammation Factors and ECM Expression in Rats of Exercise-related Renal Ischemia-reperfusion Injuries
ZHENG Mengjun1 ,CAO Jianmin2 ,GUO Xian2 ,ZHOU Haitao3
(1. Sports College,Jiangnan University ,Wuxi 214000,Jiangsu,China;
2. Sport Science College of Beijing Sport University ,Beijing 100084,China;
3. Biochemical Engineering College of Beijing Union University ,Beijing 100023,China)
Abstract:Objective:To study the protective effects of tonic medicine on kidney inflammation factors and extracellular ma- trix ( ECM ) expression in rats of exercise-related renal ischemia-reperfusion injuries. Methods:75 male Wistar rats at the age of 7 weeks were divided into 4 groups randomly:control group( C group,n = 12) ,general training group( M group,n =
12) ,overtraining group( OM group,n = 24) ,tonic medicine + overtraining group( TM group,n = 24) ,the Rats in M , OM ,TM groups were assigned to 8 weeks of swimming training,7 days a week. Professional gavage were given once a day. The Rats of TM group were given with compound Chinese herbal medicine( The primary elements were Chinese drugs for invigoration,such as Astragalus,Rhodiola rosea,Salviae miltiorrhizae,Sophora flavescens) ,gavages( ig) dose was 1 g
· kg - 1 ;ig volume was 5 mL·kg - 1 ,and other groups were given the normal saline. 24 hours after the last training,w e ob-
served the glomerular ECM deposition by the PAS stain;detected the renal tissue of TNF-α,IL-1β,IL-6,IL-18 protein by immunohistochemistry and detected the expression of TNF-α mRNA,IL-1β mRNA,IL-6 mRNA,IL-18 mRNA,TGF-β1 mRNA gene in renal tissue by RT-PCR method. Results:1)8 weeks after the experiment,the glomerular ECM deposition of C group and M group appeared normal and showed no differences( P > 0. 05);OM group was significantly higher than that in C group and M group( P < 0. 01) ,TM group was significantly lower than that of group OM ( P < 0. 05). 2) OM
收稿日期: 2015-03-04; 修回日期: 2015-03-29
基金项目: 国家体育总局课题(2013A101)。
作者简介: 郑孟君(1980—) ,女,讲师,硕士,主要研究方向为体育教学与运动训练。通信作者: 周海涛(1976—) ,男,副教授,硕士,主要研究方向为运动性疲劳与恢复。
第 3 期 郑孟君,等: 补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子及 ECM 表达的影响 ·69·
group and TM group blood urea nitrogen and serum creatinine were significantly higher than that of C group( P < 0. 01), TM group was significantly lower than that of group OM ( P < 0. 05);the expression of renal TNF-α,IL-1 β,IL-6 and IL- 18 protein in OM group and TM group was significantly higher than that in C group( P < 0. 01) ,TM group was significant- ly lower than that of group OM ( P < 0. 05);the expression of renal TNF- α mRNA,IL-1 β mRNA,IL-6 and IL-18 mRNA in TM group and OM group was significantly higher than that of C group( P < 0. 01) ,TM group was lower than of OM group( P < 0. 05);the expression of renal TGF-β1mRNA in TM group and OM group was significantly higher than that of C group( TM ,P < 0. 05;OM ,P < 0. 01) ,TM group was significantly lower than of OM group( P < 0. 05). Conclusion: 8 weeks overtraining caused the movement of rat renal ischemia reperfusion and increased the deposition of ECM . Tonic medicine can restrain the expression of renal tissue TGF-β1 during exercise-related renal ischemia-reperfusion injury in- duced by overtraining,w hich may be achieved by inhibiting the expression of IL-1,TNF-α,IL-6,IL-18 in renal tissue. Fi- nally it decreased the synthetic and increased the decomposition of ECM ,reduced the damage and the histopathological changes,as a result,Tonic medicine can protect the rats from exercise-related renal ischemia-reperfusion injury.
Key words:tonic medicine;exercise-related renal ischemia-reperfusion injury;inflammation factors;extracellular matrix
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